产品简介

SP2/0-Ag14为小鼠骨髓瘤(Myeloma)细胞系常被简称为SP2/0) e,。该细胞是由绵羊红细胞免疫的BALB/c小鼠脾细胞和P3X63Ag8骨髓瘤细胞(Hybridoma B lymphocyte)融合而得，H-2d抗原阳性。该细胞鼠痘病毒阴性，不分泌免疫球蛋白，对20 μg/ml的8-氮鸟嘌呤有抗性，对HAT比较敏感。该细胞系生长稳定，繁殖旺盛，是构建鼠源杂交瘤单克隆抗体常用的亲本细胞。细胞融合时，常作为可用HAT培养基筛选，具有永生化特性的骨髓瘤细胞用于单克隆抗体杂交瘤的构建。

SP2/0-Ag14细胞系(SP2/0-Ag14 Cell Line)为小鼠骨髓瘤细胞，半悬浮半贴壁生长，培养条件为含10%FBS的DMEM或RPMI-1640完全培养液。

包装

保存

无菌包装。完全培养液或液氮冻存。

参考文献

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2、Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293

3、Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

4、Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022