产品简介

HL-60细胞系源自一位患有急性早幼粒细胞性白血病的36岁白人女性的外周血，由Collins SJ分离建立。可自发分化，或在丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸的刺激下发生分化。PMA刺激后可分泌TNF-α。该细胞具有吞噬活性和趋化反应，癌基因myc阳性，并表达补体受体和FcR。该细胞系生长稳定，繁殖旺盛，细胞群体倍增时间36-48小时。

HL-60细胞系(HL-60 Cell Line)为人早幼粒急性白血病细胞，悬浮生长，培养条件为含10%FBSD的IMDM完全培养液。

包装

保存

无菌包装。完全培养液或液氮冻存。

参考文献

1、Gallagher R, et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979. PubMed:288488

2、Collins SJ, et al. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75: 2458-2462, 1978. PubMed: 276884

3、Collins SJ, et al. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270: 347-349, 1977. PubMed: 271272